Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice

Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

BACKGROUND Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/aneuploid prototrophs (2) available strain auxotrophies must be paired with available repair plasmi...

Design and Construction of ctxB-gfp-stxB Gene Cassette and Investigation of Its Expression in E. coli Bl21 (DE3)

Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies.   Materials & Methods: After prep...

Chemical synthesis and expression of a cassette adapted ubiquitin gene.

A gene encoding the yeast ubiquitin was chemically synthesized and expressed in yeast under regulatory control of the copper metallothionein (CUP1) promoter. The gene was assembled in a one-step ligation reaction from eight oligonucleotide fragments ranging in length from 50 to 64 nucleotides. To facilitate mutagenesis and gene fusion studies, eight unique 6-base-cutting restriction enzyme site...

Correctly identifying the streptothricin resistance gene cassette.

non-invasive evaluation of embryo morphological plasticity by designing of new transgenic gene cassette

abstract background: determination of transgenic embryos from non transgenic embryos sibling is an important step in producing homozygous transgenic mice. these steps need by pcr or southern blotting followed extraction of dna, but both techniques require skill and consume time. objective: the aim of this study was simulation of high accuracy method using novel enhanced green fluorescent protei...